Journal: Translational Cancer Research

Article Title: Propofol regulates the progression of hepatocellular carcinoma via the POLR2L/TGF-β signaling pathway

doi: 10.21037/tcr-23-2066

Figure Lengend Snippet: Effects of POLR2L on proliferation, invasion and migration of HCC cell lines. (A) The expression of POLR2L in HCC cell lines and normal cells was detected by qRT-PCR and WB assays. (B,C) Detection of POLR2L knockdown efficiency of siRNA in SNU-387 and MHCC-97H cells. (D,E) The proliferation ability of SNU-387 and MHCC-97H cells after POLR2L knockdown was detected by CCK-8 methods. (F-I) Transwell assay was used to detect the invasion and migration ability of SNU-387 and MHCC-97H cells after POLR2L knockdown. Magnification ×50, staining with DAPI. *, P<0.05; **, P<0.01. NC, negative control; OD, optical density; HCC, hepatocellular carcinoma; qRT-PCR, quantitative reverse transcription-polymerase chain reaction; WB, Western blotting; siRNA, small interfering RNA; CCK-8, cell counting kit-8; DAPI, 4',6-diamidino-2-phenylindole.

Article Snippet: GeneChem Co., Ltd. (Shanghai, China) designed and produced the small interfering RNA (siRNA) targeting POLR2L (si-POLR2L #1 and si-POLR2L #2) and a nontargeting control siRNA (si-NC).

Techniques: Migration, Expressing, Quantitative RT-PCR, Knockdown, CCK-8 Assay, Transwell Assay, Staining, Negative Control, Reverse Transcription, Polymerase Chain Reaction, Western Blot, Small Interfering RNA, Cell Counting

Journal: RNA Biology

Article Title: Altered Levels of Long NcRNAs Meg3 and Neat1 in Cell And Animal Models Of Huntington’s Disease

doi: 10.1080/15476286.2018.1534524

Figure Lengend Snippet: Summary of the levels of ncRNAs in different models of HD.

Article Snippet: SiRNA mediated knockdown of Neat1 and Meg3 siRNAs against mouse NEAT1 (FlexiTube GeneSolution GS66961 for Neat1, Cat no: LPM15183A) and MEG3 (FlexiTube GeneSolution GS17263 for Meg3, Cat No: GS17263) were purchased from Qiagen and used, following the protocol described by the manufacturer. siRNAs were used at a final concentration of 40nM.

Techniques: Transfection

Journal: RNA Biology

Article Title: Altered Levels of Long NcRNAs Meg3 and Neat1 in Cell And Animal Models Of Huntington’s Disease

doi: 10.1080/15476286.2018.1534524

Figure Lengend Snippet: Summary of protein and miRNA interactions of ncRNAs Meg3, Neat1, and Xist.

Article Snippet: SiRNA mediated knockdown of Neat1 and Meg3 siRNAs against mouse NEAT1 (FlexiTube GeneSolution GS66961 for Neat1, Cat no: LPM15183A) and MEG3 (FlexiTube GeneSolution GS17263 for Meg3, Cat No: GS17263) were purchased from Qiagen and used, following the protocol described by the manufacturer. siRNAs were used at a final concentration of 40nM.

Techniques:

Journal: RNA Biology

Article Title: Altered Levels of Long NcRNAs Meg3 and Neat1 in Cell And Animal Models Of Huntington’s Disease

doi: 10.1080/15476286.2018.1534524

Figure Lengend Snippet: Expression of genes in HD associated with Huntington’s disease pathway (KEGG: 05016 and PANTHER: {"type":"entrez-protein","attrs":{"text":"P00029","term_id":"124076962","term_text":"P00029"}} P00029 ) and coded for protein interacting partners of NEAT1 or MEG3.

Article Snippet: SiRNA mediated knockdown of Neat1 and Meg3 siRNAs against mouse NEAT1 (FlexiTube GeneSolution GS66961 for Neat1, Cat no: LPM15183A) and MEG3 (FlexiTube GeneSolution GS17263 for Meg3, Cat No: GS17263) were purchased from Qiagen and used, following the protocol described by the manufacturer. siRNAs were used at a final concentration of 40nM.

Techniques: Expressing

Journal: RNA Biology

Article Title: Altered Levels of Long NcRNAs Meg3 and Neat1 in Cell And Animal Models Of Huntington’s Disease

doi: 10.1080/15476286.2018.1534524

Figure Lengend Snippet: Summary of co-expressed genes of MEG3, NEAT1, and XIST.

Article Snippet: SiRNA mediated knockdown of Neat1 and Meg3 siRNAs against mouse NEAT1 (FlexiTube GeneSolution GS66961 for Neat1, Cat no: LPM15183A) and MEG3 (FlexiTube GeneSolution GS17263 for Meg3, Cat No: GS17263) were purchased from Qiagen and used, following the protocol described by the manufacturer. siRNAs were used at a final concentration of 40nM.

Techniques:

Journal: RNA Biology

Article Title: Altered Levels of Long NcRNAs Meg3 and Neat1 in Cell And Animal Models Of Huntington’s Disease

doi: 10.1080/15476286.2018.1534524

Figure Lengend Snippet: MEG3, NEAT1 and XIST interacting protein enriched with HD pathway.

Article Snippet: SiRNA mediated knockdown of Neat1 and Meg3 siRNAs against mouse NEAT1 (FlexiTube GeneSolution GS66961 for Neat1, Cat no: LPM15183A) and MEG3 (FlexiTube GeneSolution GS17263 for Meg3, Cat No: GS17263) were purchased from Qiagen and used, following the protocol described by the manufacturer. siRNAs were used at a final concentration of 40nM.

Techniques:

Journal: RNA Biology

Article Title: Altered Levels of Long NcRNAs Meg3 and Neat1 in Cell And Animal Models Of Huntington’s Disease

doi: 10.1080/15476286.2018.1534524

Figure Lengend Snippet: Transcription factors that bind within 5 Kb upstream sequences of NEAT1, MEG3, and XIST.

Article Snippet: SiRNA mediated knockdown of Neat1 and Meg3 siRNAs against mouse NEAT1 (FlexiTube GeneSolution GS66961 for Neat1, Cat no: LPM15183A) and MEG3 (FlexiTube GeneSolution GS17263 for Meg3, Cat No: GS17263) were purchased from Qiagen and used, following the protocol described by the manufacturer. siRNAs were used at a final concentration of 40nM.

Techniques: Sequencing

Journal: Kidney international

Article Title: TLR9 activation induces aberrant IgA glycosylation via APRIL- and IL-6-mediated pathways in IgA nephropathy

doi: 10.1016/j.kint.2019.08.022

Figure Lengend Snippet: (A) CpG-ODN supplementation enhanced production of IL-6. CpG-ODN enhanced gene expression of APRIL. Western-blot analysis demonstrated that CpG-ODN increased protein levels of APRIL. Results were evaluated densitometrically. Stimulation with CpG-ODN induced production of IgA and Gd-IgA1. Bars represent the mean±SEM. *P<0.05, **P<0.01. (B) APRIL siRNA knock-down reduced IL-6-induced overproduction of IgA and Gd-IgA1. *P<0.05, **P<0.01. (C) IgA1-producing cells stimulated with recombinant APRIL produced more IgA and Gd-IgA1. (D) IgA1-secreting cells were incubated with anti-IL-6 antibody after CpG-ODN stimulation. The enhanced production of IgA and Gd-IgA1 induced by CpG-ODN was reduced by anti-IL-6 antibody and by siRNA knock-down of APRIL. PBS, Phosphate Buffered Saline.

Article Snippet: Human IgA1-secreting cells were cultured in 24-well culture plates and transfected with APRIL -specific siRNA (GS8741, Qiagen) or siRNA non-targeting control (GS10673, Qiagen) using the transfection protocol according to the manufacturer’s instructions.

Techniques: Gene Expression, Western Blot, Knockdown, Recombinant, Produced, Incubation, Saline